Atlas-based automated detection of swim bladder in Medaka embryo

Diane Genest (1) , Marc Léonard (2) , Jean Cousty (1) , Noémie de Crozé (3) , Hugues Talbot (1, 4, 5)

1 LIGM - Laboratoire d'Informatique Gaspard-Monge
2 L'Oréal Recherche - L'Oréal Recherche France
3 RCO - Régulations cellulaires et oncogenèse
4 CVN - Centre de vision numérique
5 OPIS - OPtimisation Imagerie et Santé

Fish embryo models are increasingly being used both for the assessment of chemicals efficacy and potential toxicity. This article proposes a methodology to automatically detect the swim bladder on 2D images of Medaka fish embryos seen either in dorsal view or in lateral view. After embryo segmentation and for each studied orientation, the method builds an atlas of a healthy embryo. This atlas is then used to define the region of interest and to guide the swim bladder segmentation with a discrete globally optimal active contour. Descriptors are subsequently designed from this segmentation. An automated random forest clas-sifier is built from these descriptors in order to classify embryos with and without a swim bladder. The proposed method is assessed on a dataset of 261 images, containing 202 embryos with a swim bladder (where 196 are in dorsal view and 6 are in lateral view) and 59 without (where 43 are in dorsal view and 16 are in lateral view). We obtain an average precision rate of 95% in the total dataset following 5-fold cross-validation.

Mots clés: Toxicological screening; Segmentation; Mathematical morphology; Affine registration; Biomedical atlas; Descriptor design; Random forest classification

Domaines: Intelligence artificielle [cs.AI]; Mathématique discrète [cs.DM]; Vision par ordinateur et reconnaissance de formes [cs.CV]; Traitement des images [eess.IV]

Diane Genest, Marc Léonard, Jean Cousty, Noémie de Crozé, Hugues Talbot. Atlas-based automated detection of swim bladder in Medaka embryo. ISMM 2019 - International Symposium on Mathematical Morphology and Its Application to Signal and Image Processing, Bernhard Burgeth; Andreas Kleefeld; Benoit Naegel; Nicolas Passat; Benjamin Perret, Jul 2019, Saarbrücken, Germany. pp.496-507, ⟨10.1007/978-3-030-20867-7_38⟩. ⟨hal-02019658⟩