Electrochemical Properties of Model Compounds of Nicotinamide-adenine-dinucleotide in Aqueous Solution

Daniel R. Thevenot (1) , Gilbert Hammouya (1)

1 LEE - Laboratoire d'Energétique Electrochimique

The reduction behavior of N1-methyl nicotinamide iodide has been studied by drop-time controlled polarography. ln highly acidic media (below pH 3) two pH dependent waves were observed (I and l') the latter having an abnormally high limiting current. ln the pH range of 3-10, two waves were observed (Il and III), the former being pH-independent whereas the E1/2 of the latter shifted - 60 mV per pH unit. Evidence is presented for a chemical association step, possibly a dimerization occurring between reduction steps II and III. Reoxidation of the reduction product of wave II was obtained at 740 mV above the potential of the wave II by cyclic voltammetry on stationary vitreous carbon electrodes. This reoxidation peak is also pH independent.

Domains: Analytical chemistry; Biochemistry [q-bio.BM]; Biophysics

Daniel R. Thevenot, Gilbert Hammouya. Electrochemical Properties of Model Compounds of Nicotinamide-adenine-dinucleotide in Aqueous Solution. Biological Aspects of ElectrochemistryExperientia Suppl., 18, pp.631 - 645, 1971. ⟨hal-01096351⟩