Characterization of a protease of a feather-degrading Microbacterium species. - École des Ponts ParisTech Accéder directement au contenu
Article Dans Une Revue Letters in Applied Microbiology Année : 2004

Characterization of a protease of a feather-degrading Microbacterium species.

R. C. S. Thys
  • Fonction : Auteur
F. S. Lucas
A. Riffel
  • Fonction : Auteur
A. Brandelli
  • Fonction : Auteur

Résumé

AIMS: To characterize a new feather-degrading bacterium. METHODS AND RESULTS: The strain kr10 producing a high keratinolytic activity when cultured on native feather broth was identified as Microbacterium sp., based on phenotypical characteristics and 16S rDNA sequence. The bacterium presented optimum growth and feather-degrading activity at pH 7.0 and 30 degrees C. Complete feather degradation was achieved during cultivation. The keratinase was partially purified by gel filtration chromatography. It was optimally active at pH 7.0 and 55 degrees C. The enzyme was inhibited by 1,10-phenanthroline, EDTA, p-chloromercuribenzoic acid, 2-mercaptoethanol and metal ions like Hg(2+), Cu(2+) and Zn(2+). SIGNIFICANCE AND IMPACT OF THE STUDY: A new Microbacterium sp. strain was characterized presenting high feather-degrading activity, which appears to be associated to a metalloprotease-type keratinase. This micro-organism has enormous potential for use in biotechnological processes involving keratin hydrolysis.
Fichier non déposé

Dates et versions

hal-00814497 , version 1 (17-04-2013)

Identifiants

Citer

R. C. S. Thys, F. S. Lucas, A. Riffel, P. Heeb, A. Brandelli. Characterization of a protease of a feather-degrading Microbacterium species.. Letters in Applied Microbiology, 2004, 39 (2), pp.181-6. ⟨10.1111/j.1472-765X.2004.01558.x⟩. ⟨hal-00814497⟩
55 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More