Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Optimization of large-volume injection for the determination of polychlorinated biphenyls in children's fast-food menus by low-resolution mass spectrometry.

Abstract : This study includes the determination of five indicator polychlorinated biphenyls (PCBs) (52, 101, 153, 138, and 180), six non-ortho PCBs (35, 80, 81, 77, 126, and 169), and two mono-ortho PCBs (28 and 118) in fast food for children. A freeze-dried sample of 10 g is extracted by using pressurized n-hexane in two 5 min cycles at 120 degrees C and 100 mbar. Fatty extracts were cleaned up by means of acetonitrile/n-hexane partitioning and gel-permeation chromatography. The fractionation of non-ortho, mono-ortho, and indicator PCBs was made on graphitized carbon solid-phase extraction cartridges by using n-hexane, n-hexane/toluene (99:1, v/v), and toluene as elution solvents. Gas chromatography coupled to tandem mass spectrometry and large-volume injections with a programmed-temperature vaporizer (PTV-LV) were used to increase sensitivity and selectivity of the PCB determination. The PTV-LV injection settings, that is, vaporizing temperature, vaporizing time, and purge flow, were optimized by using a central composite design. A 15-40 times increased sensitivity was reached as compared with that obtained with the conventional 1 microL splitless injection. The limits of detection achieved were between 0.3 and 1.2 pg/g, and repeatability data, as relative standard deviation varied, ranged from 2 to 9% for the 0.05 ng/mL PCB level.
keyword : PCBs
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-enpc.archives-ouvertes.fr/hal-00750959
Contributeur : Emilie Caupos <>
Soumis le : lundi 12 novembre 2012 - 17:54:30
Dernière modification le : samedi 7 avril 2018 - 01:19:05

Lien texte intégral

Identifiants

Collections

Citation

Francesc A Esteve-Turrillas, Emilie Caupos, Isabel Llorca, Agustín Pastor, Miguel de la Guardia. Optimization of large-volume injection for the determination of polychlorinated biphenyls in children's fast-food menus by low-resolution mass spectrometry.. Journal of Agricultural and Food Chemistry, American Chemical Society, 2008, 56 (6), pp.1797-803. ⟨10.1021/jf073141u⟩. ⟨hal-00750959⟩

Partager

Métriques

Consultations de la notice

253