Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.

Nicolas Radomski (1) , Françoise S Lucas , Régis Moilleron (1) , Emmanuelle Cambau , Sophie Haenn , Laurent Moulin

1 LEESU - Laboratoire Eau, Environnement et Systèmes Urbains

A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

Domaines: Sciences de l'environnement

Nicolas Radomski, Françoise S Lucas, Régis Moilleron, Emmanuelle Cambau, Sophie Haenn, et al.. Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.. Applied and Environmental Microbiology, 2010, 76 (21), pp.7348-51. ⟨10.1128/AEM.00942-10⟩. ⟨hal-00676401⟩