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Journal articles
Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast
Abstract : Calcium (Ca2+) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca2+- and redox-related signalling. This protein, designated as calredoxin (CRX), combines four Ca2+-binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 angstrom resolution, revealed an unusual calmodulin-fold of the Ca2+-binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys peroxiredoxin (PRX1). Ca2+-binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca2+-dependent 'sensor-responder' proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation.
Cited literature [70 references]
https://hal.archives-ouvertes.fr/hal-01412644
Contributor : Jacqueline Martin-Laffon <>
Submitted on : Wednesday, May 27, 2020 - 1:03:19 PM
Last modification on : Thursday, November 12, 2020 - 7:04:04 PM
Contributor : Jacqueline Martin-Laffon <>
Submitted on : Wednesday, May 27, 2020 - 1:03:19 PM
Last modification on : Thursday, November 12, 2020 - 7:04:04 PM
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